Hi everyone,
Please check this thread Mini-MACSA, anyone??? - Page 4 - Marine Aquariums of South Africa and let's have your thoughts - who would be willing to participate,. and perhaps host some reefers for a weekend in March 2012?
Hennie
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Abstract Zoanthids (Anthozoa, Hexacorallia) are colonial anemones that contain one of the deadliest toxins ever discovered, palytoxin (LD50 in mice 300 ng/kg), but it is generally believed that highly toxic species are not sold in the home aquarium trade. We previously showed that an unintentionally introduced zoanthid in a home aquarium contained high concentrations of palytoxin and was likely responsible for a severe respiratory reaction when an individual attempted to eliminate the contaminant colonies using boiling water. To assess the availability and potential exposure of palytoxin to marine aquarium hobbyists, we analyzed zoanthid samples collected from local aquarium stores for palytoxin using liquid chromatography and high resolution mass spectrometry and attempted to identify the specimens through genetic analysis of 16S and cytochrome c oxidase 1 (COI) markers. We found four specimens of the same apparent species of zoanthid, that we described previously to be responsible for a severe respiratory reaction in a home aquarium, to be available in three aquarium stores in the Washington D.C. area. We found all of these specimens (n = 4) to be highly toxic with palytoxin or palytoxin-like compounds (range 0.53.5 mg crude toxin/g zoanthid). One of the most potent non-protein compounds ever discovered is present in dangerous quantities in a select species of zoanthid commonly sold in the home aquarium trade. Introduction In the late 1950's, Dr. Albert H. (Hank) Banner began a program at the University of Hawaii to search for the elusive cause of ciguatera fish poisoning. Dr. Philip Helfrich, a young researcher hired by Banner, began this search by investigating an entry in the Hawaiian dictionary for the limu-make-o-Hana (rough translation deadly seaweed of Hana). This legend dates back to Hawaiian antiquity with tales of Shark Gods, sacred pools, and a seaweed when applied to a warrior's spear would bring sure death to their enemies. The pool became kapu or taboo to the local Hawaiians and it was said that an ill fate would befall anyone who disturbed the sacred site. Every legend holds some basis in fact, and in 1961, Helfrich, accompanied by graduate student John Shupe, tracked down the fabled pool near the village of Mu′olea on the island of Maui and introduced to the world a new species of cnidarian zoanthid (colonial anemone) known as Palythoa toxica. This research led to the discovery of palytoxin (PLTX), one of the most potent natural products ever discovered. Although much of the structural elucidation of palytoxin would be determined from less toxic, but far more abundant, species such as Palythoa tuberculosa none were ever found to be as potent as the samples collected from the tidepools at Mu′olea. Now the legendary limu appears to be exacting its ancient curse once again, but this time upon unsuspecting marine home aquarists. In 2007, we assisted the Georgia poison center in an investigation into a potential dermal intoxication with palytoxin from zoanthids in a home aquarium. During the course of the investigation, we learned of another marine aquarium hobbyist in Virginia who had recently experienced a severe respiratory reaction while trying to eradicate brown non-descript colonies of zoanthids that had arrived as hitchhikers with live rock and were overgrowing more desirable organisms in the tank [account can be found at: http://www.reefcentral.com/forums/showthread.php?t=1083843 accessed 12/09/10]. A sample (here called Virginia zoanthid or VAZOA) was found to contain high levels of palytoxin (600 µg crude toxin/g wet zoanthid). Zoanthids are a notoriously problematic taxonomic group and it proved difficult at the time to identify to the species level the sample from the home aquarium in Virginia. Histologic evaluation of preserved polyps could only confirm that it was a Palythoa sp. (D. Fautin, University of Kansas, personal communication). In an attempt to determine the prevalence of palytoxin in aquarium store zoanthids, we purchased several colonies from local aquarium stores and analyzed them for palytoxin. We further performed a molecular analysis in an attempt to identify the colonies to the species level. Conclusions Some of the deadliest toxins known to man come from the sea. Maitotoxin produced by tropical single celled dinoflagellates of the genus Gambierdiscus has an intraperitoneal (IP) lethality (in mice) of 50 ng/kg. Meaning 1 gram of toxin can kill 1 billion mice. It is the most potent non-peptide natural product ever discovered. Palytoxin comes in second with an IP mouse lethality of 300 ng/kg. Even though our typical sample size for zoanthids collected in this study was small (0.20.8 g/sample), we calculate that we extracted enough crude toxin from these combined samples to kill 300,000 mice (approx. 2 mg crude toxin calculated by HPLC, standard mouse size of 20 g). Palytoxins, along with several structurally related compounds (e.g. ostreocins and ovatoxins produced by select marine dinoflagellates) have garnered significant recent attention due to the fact that they are one of the few marine toxins that pose a risk to humans through ingestion (consumption of contaminated seafood), inhalation (exposure to palytoxin containing aerosols), and dermal (exposure to select marine zoanthids) routes of exposure. Numerous poisonous and venomous animals are available at your local pet store. Animals imported for the pet/aquarium trade are regulated based on their CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) status and not based on potential toxicity. Venomous lionfish (family Scorpaenidae), and alkaloid containing poison dart frogs (family Dentrobatidae) are commonplace. Although more rare, highly venomous (with tetrodotoxin) blue ringed octopuses (Hapalochlaena spp.) can occasionally be found in home aquaria. Furthermore, importation of whole tetrodotoxin containing puffer fish (family Tetraodontidae), which are prohibited for importation (whole) as food, have no such restrictions if they are imported as pets. There is great debate among marine aquarium enthusiasts on the hazards of keeping zoanthids. Some literature warns that all zoanthids are toxic, while many hobbyists claim that they have been handling these organisms for years without incident (on-line accounts can be found at sites such as www.reefcentral.com). Well documented cases of human poisonings due to exposure to zoanthids in home aquaria are limited, but detailed exposure accounts with confirmation of toxin presence do exist for inhalational and dermal exposures. Additional case reports based on symptoms alone can also be found in the literature. This is the first report to our knowledge that documents both high PLTX presence and phylogenetic identity for commercially available zoanthids from aquarium stores. It is often difficult to determine the species, let alone geographic origin, for many of the organisms sold in the home aquarium trade. In the case of zoanthids, most specimens are sold under common names such as button polyps, sun polyps, or yellow/orange or green zoas; furthermore, many enthusiasts and websites selling zoanthids use colloquial names such as watermelon, scorpion, people eaters, sunset palys or zoas, etc. Discussions with the owner of aquarium store #1 revealed that he typically acquired zoanthids though the purchase of mixed containers of frags (i.e. coral or rock fragments containing zoanthid colonies) from either importers or wholesalers who typically reported them to be wild caught and of Indo-Pacific origin but with no other accompanying documentation confirming the geographic origin or identity of the specimens. Making the issue of tracing the origin of commercial zoanthids more difficult is the fact that many marine aquarium enthusiasts commonly fragment their colonies and further exchange them outside of retail sale. All of this uncertainty makes it difficult to provide a concise message to aquarium hobbyists on the hazards of keeping this group of organisms. During this investigation, we found that many of the zoanthids commonly sold in the home aquarium trade are non-toxic or weakly-toxic, but a highly toxic variety of Palythoa (possibly P. heliodiscus or P. toxica) is indeed available. It often occurs as a tank contaminant and can be unintentionally introduced with more desirable species or on live rock. Unfortunately, due to the unavailability of molecular data for P. toxica (the limu make o Hana from Hawaiian folklore), we could not determine its relationship to either the toxic specimens from our study or to P. heliodiscus, which available molecular data suggests our toxic specimens are. Hopefully, one day the type location on the island of Maui will be made available for sampling again and the true identity of P. toxica can be determined. Regardless, we have shown that a Palythoa sp. just as toxic as the legendary limu can not only be found outside of the tidepools at Mu′olea, it is available in retail commerce and present in home aquaria, with the owners often unaware of the deadly poisons they are being exposed to. |
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A satellite that will map the saltiness of seawater has gone in orbit. Data from the Aquarius/SAC-D spacecraft will help scientists understand better the processes that drive ocean circulation and the movement of freshwater around the planet. . . Scientists have been able to measure ocean salinity for decades by lowering instruments from ships or by deploying robotic floats, but the technology to sense this property from orbit is a recent innovation. Salinity is of interest to researchers because it is both a determinant of ocean behaviour and a tool to diagnose what might be happening in the climate system. Aquarius carries three high-precision radio receivers that will record the natural microwave emissions coming up off the water's surface. These emissions vary with the electrical conductivity of the water - a property directly related to how much dissolved salt it is carrying. Together with temperature, salinity will define water density, and density and wind are the wheels of ocean circulation - the means by which Earth moves much of the energy it receives from the Sun around the globe . . Our goal is to retrieve salinity at 0.2 parts per thousand," said Aquarius Principal Investigator Gary Lagerloef of Earth & Space Research in Seattle. "That's the equivalent concentration if you were to take a dash of salt about the same as one-eighth of teaspoon and put it in a gallon of water. That works out to about one millilitre of salt in six litres of water. That's the amount of salinity change that Aquarius will be able to observe from month to month over any part of the ocean. |
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Hi Hennie Me and my wife made the decision to chuck the TV and replace it with a 1,8L x 0,8W x 0,7D Reef aquarium. The tank is still in fabrication and we have some .lets say difference in opinion about the location of the equipment:blush:. It would be great to have all the stuff outside in a shed of some sort and only the tank in the room. Best regards Martin |
| Do you perhaps know if it would be possible to check Ammonia and NO2 with a control system? |
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Hi Hennie Would you mind posting it? Im still finding my way on the site and T&C of the site I would love to see the people comment on this topic and in the mean time Ill read the T&Cs and keep you posted on the control system info I can find. Regards Martin |

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In late June 2008, the waters and shores at the Qingdao venue hosting the Olympic sailing regatta experienced a massive green tide covering about 600 sq km. Lasting over two weeks, it took more than 10,000 people to clean up, removing over one million tonnes of algae from the beach and coast... ...Within 10 days, these patches had moved away from the coast and into the Yellow Sea, covering 1,200 sq km, and impacting about 40,000 sq km of ocean, making it the largest algal boom, or green tide, ever recorded. |
Loss of international connectivity attributed to SAT-3 cable![]() Hetzner customers, together with most SA Internet users, are currently experiencing severe international connectivity problems since Wednesday, 20 January 2010. This means that browsing to international websites and receiving email from international sources has proven problematic. This issue is a result of a SAT-3 cable break on the London-Bellville link which continues to cause slow international connectivity. Hetzner is not able to anticipate or prevent issues relating to international connectivity accessed via the SAT-3 cable. Your local access partner or ISP (e.g. M-Web) gives you access to international bandwidth and it is this connectivity path that has been compromised by the SAT-3 cable break. Telkom SA is conducting major maintenance on the fault, with the maintenance window running from 20-22 January and again from 29 January to 1 February. During this maintenance window, most South African Internet users will experience intermittent high latency to international locations. |






